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Altered Behavioral Performance in the Cell‐specific HIF‐1α and HIF‐2α Deficient Mice
Author(s) -
Xu Kui,
Sun Xiaoyan,
Radford Thomas,
LaManna Joseph
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.682.4
Subject(s) - spontaneous alternation , hypoxia (environmental) , endocrinology , medicine , knockout mouse , chemistry , cognition , neuroscience , biology , hippocampus , oxygen , receptor , organic chemistry
Hypoxia‐inducible factors (HIFs, including HIF‐1 and HIF‐2) are transcriptional regulators that mediate hypoxic adaptation and plays physiologically important roles in oxygen transport. We compared the voluntary wheel running over a 24‐h period in the cell‐specific (neuronal or glial) HIF‐1α or HIF‐2α knockout (KO) mice and their wild‐type (WT) controls. Cognitive function was measured using a Y‐maze test. The running distances in the neuronal HIF‐1α KO mice were similar compared to the WT controls (9.2 ± 2.1 km, n = 30 vs. 9.3 ± 3.0, n = 20), whereas the running distances in the glial HIF‐1α KO mice were significantly reduced (7.0 ± 3.4 km, n = 18). However, the running distances in the neuronal HIF‐2α KO mice (10.9 ± 2.8 km, n = 36) were significantly increased compared to the WT controls (7.7 ± 3.2 km, n = 22) and the glial HIF‐2α KO mice (7.8 ± 2.2 km, n = 3). In the Y‐maze test, all groups had a comparable alternation rate (indicative of sustained cognition), with the lowest alternation rate in the glial HIF‐2α KO mice (%, 53 ± 9, n = 3) and the highest alternation rate in the HIF‐2 WT controls (%, 63 ± 9, n = 17). Our data demonstrated that the voluntary exercise activity and cognitive function were altered in the mice with neuronal or glial deficiency of HIF‐1α or HIF‐2α, suggesting the effect of HIF‐1α or HIF‐2α gene deletion on voluntary exercise activity and cognitive function may be cell‐specific.