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Hyperbaric oxygen preconditioning attenuated high‐altitude pulmonary edema via heat shock protein 70 overexpression
Author(s) -
Lin HungJung,
Chang ChingPing,
Tsai MingChe
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.680.3
Subject(s) - edema , aquaporin 1 , medicine , lung , pulmonary edema , hsp70 , effects of high altitude on humans , hyperbaric oxygen , anesthesia , heat shock protein , infiltration (hvac) , hypobaric chamber , high altitude pulmonary edema , immunology , chemistry , anatomy , biochemistry , mechanical engineering , physics , water channel , engineering , inlet , gene , thermodynamics
Evidence has indicated that reducing pulmonary injury by HBO 2 P (hyperbaric oxygen preconditioning) in rats during simulated high‐altitude exposure (HAE). However, underlying mechanisms remains unclear. Rats were randomly divided into four groups: the non‐HBO 2 P+non‐HAE group, the non‐HBO 2 P+HAE group, the HBO 2 P+HAE group, and the HBO 2 P+HSP‐70 antibodies (Abs)+HAE group. The HBO 2 P groups were given 100% O 2 at 2.0 absolute atmospheres for 1 hour per day for 5 consecutive days. The HAE groups were exposed to simulated HAE (9.7% O 2 at 0.47 absolute atmospheres) in a hypobaric chamber for 3 days, HSP‐70 Abs were intravenously injected 24 hours before the HAE experiments. In non‐HBO 2 P+HAE group, the animals displayed higher scores of alveolar edema, neutrophil infiltration, and hemorrhage, and lower levels of aquaporin (AQP) 1 and AQP 5 proteins and mRNA expression in the lung compared with those of non‐HBO 2 P+non‐HAE controls. The increased lung injury scores and the decreased levels of both AQP1 and AQP5 proteins and mRNA expression in the lung caused by HAE was significantly reduced by HBO 2 P+HAE. Furthermore, HSP70 Abs, in addition to reducing lung HSP70 proteins, significantly attenuated the beneficial effects of HBO 2 P in HAE. Our results suggest that high‐altitude lung edema can be prevented by HSP‐70‐mediated HBO 2 P in rats.