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The Isolation of Volatile Organic Compounds as Noninvasive Early Markers of Pulmonary Oxygen Toxicity in Swine
Author(s) -
Senese Angela,
Cronin William,
Mahon Richard,
Regis David,
Hall Aaron
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.678.2
Subject(s) - oxygen toxicity , oxygen , toxicity , medicine , anesthesia , chemistry , zoology , biology , organic chemistry
Purpose Pulmonary oxygen toxicity (PO 2 T) is a concern for any patient or warfighter exposed to oxygen enriched atmospheres for an extended amount of time. While current practice is to limit increased oxygen exposure; there are clinical and operational scenarios where higher oxygen levels must be used and PO 2 T becomes a concern. The purpose of the current study is to develop a non‐invasive breath exhalate based biomarker which can detect PO 2 T prior to clinical manifestations of the disease. Methods Male Yorkshire swine (20‐30 kg) were placed into custom plexi‐glass runs and randomized to air (n=10) or 100% oxygen (n=10) for 72 hours. Breath exhalate samples, arterial blood gas samples, and vital signs were assessed every 12 hours. At the end of the 72 hour exposure animals were euthanized and the lungs were processed for histological injury and wet/dry ratios. Results Swine exposed to the oxygen enriched atmosphere developed a variable pulmonary injury consistent with PO 2 T. Histologically there was evidence of pulmonary lymphatic congestion, epithelial sloughing, and neutrophil transmigration. This evidence was supported by a trend of increased mean wet/dry ratio from 5.23g in the air group to 6.46g in the oxygen group (students t test, p=0.08). Also, the PaO2/FiO2 ratio trended to a decreased mean of 447 mm Hg in the air group to 377 mm Hg in the oxygen group (students t test, p=0.08). Breath exhalate sample analysis identified six Volatile Organic Compounds (VOCs) that were predictive of PO 2 T.