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Effect of simulated air dive and decompression sickness on the plasma proteome of rats
Author(s) -
Guerrero Francois,
Pichereau Vianney,
Lautridou Jacky,
Buzzacott Peter,
Wang Qiong,
Driad Sabrina,
Mazur Aleksandra,
Lambrechts Kate,
Théron Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.678.17
Subject(s) - proteome , decompression sickness , decompression , blood proteins , blood pressure , medicine , chemistry , tandem mass spectrometry , physiology , mass spectrometry , biochemistry , chromatography
Decompression sickness (DCS) is a poorly understood systemic disease caused by inadequate desaturation following a reduction in ambient pressure. Although recent studies highlight the importance of circulating factors, the available data are still puzzling. In this study we aimed to identify proteins and biological pathways involved in the development of DCS in rats. Eighteen male Sprague‐Dawley rats were subjected to a simulated dive to 1000 kPa absolute pressure and divided into two groups: without or with clinical symptoms of DCS. A third control group remained at atmospheric pressure. Venous blood was collected one hour after hyperbaric exposure and the plasma proteomes from six individuals per group were analyzed by using a two dimensional electrophoresis based proteomic strategy. Quantitative analysis with SameSpot software identified five protein spots with abundances significantly changed (p < 0.05) between the tested conditions. Three appeared increased in DCS animals but displayed only weak changes (approx. 1.5 fold). By contrast, two protein spots dramatically decreased (i.e. quite disappeared) in animals displaying severe DCS symptoms. Identification of these proteins is currently underway using tandem mass spectrometry. This will allow for a better understanding of the molecular basis of DCS.