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Effects of normoxic and hypoxic exercise regimens on monocyte‐promoted thrombin generation in sedentary men
Author(s) -
Wang JongShyan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.677.7
Subject(s) - endocrinology , monocyte , medicine , proinflammatory cytokine , thrombin generation , chemistry , oxidative stress , tissue factor , catecholamine , epinephrine , thrombin , inflammation , coagulation , platelet
This study investigates how normoxic and hypoxic exercise training affect procoagulant monocyte‐derived microparticle (MDMP) formation and monocyte‐promoted thrombin generation (TG) under severe hypoxic stress. A total of 40 sedentary males were randomized to perform either normoxic (NET; 21%O 2 , n=20) or hypoxic (HET; 15%O 2 , n=20) exercise training (60%VO 2max ) for 30 min/day, 5 days/week for 5 weeks. At rest and immediately after hypoxic exercise test (HE, 100W under 12%O 2 for 30 min), the MDMP characteristics and dynamic TG were measured by flow cytometry and thrombinography, respectively. Before the intervention, acute HE increased coagulant factor V (FV)/FVIII‐rich, phosphatidylserine (PS)‐exposed, and tissue factor (TF)‐expressed MDMP levels and enhanced the peak height and rate of TG under treatment of monocyte with oxidative‐low density lipoprotein, which were accompanied by elevated concentrations of norepinephrine/epinephrine, myeloperoxidase, and lnterleukin‐6 in plasma. However, both NET and HET decreased the FV/FVIII‐rich, PS‐exposed, and TF‐expressed MDMP levels and the peak height and rate of TG in monocyte‐rich plasma (MRP) following HE. However, HET significantly attenuated the extents of HE‐enhanced procoagulant MDMP formation, dynamic TG in MPR, and plasma catecholamine, peroxide, and proinflammatory cytokine levels, compared to NET did. Hence, HET effectively suppresses monocyte‐mediated TG by decreasing procoagulant MDMP formation under severe hypoxic stress.