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Higher‐Volume Resistance Exercise Preferentially Increases Androgen Receptor‐DNA Binding and Canonical Wnt/β‐Catenin Signaling in Resistance‐Trained Men
Author(s) -
Spillane Micheil,
Schwarz Neil,
Willoughby Darryn
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.675.28
Subject(s) - endocrinology , medicine , androgen receptor , testosterone (patch) , dihydrotestosterone , analysis of variance , resistance training , leg press , skeletal muscle , androgen , chemistry , prostate cancer , hormone , cancer
The purpose of the study was to determine the effect of single bouts of lower‐body, lower‐volume (LBLV) and total‐body, higher‐volume (TBHV) resistance exercise on serum testosterone (T) levels and the subsequent effects on muscle T, dihydrotestosterone (DHT), androgen receptor (AR) protein content, and AR‐DNA binding. A secondary purpose was to determine the effects on serum Wnt4 levels and skeletal muscle β‐catenin content. In a randomized cross‐over design, venous blood was obtained in resistance‐trained males immediately before and after, and after 0.5, 1, 2, 3, and 24 hr following a single bout of exercise. Muscle samples were obtained immediately before and 3 and 24 hr after exercise. Exercise bouts consisted of a LBLV and TBHV protocol, and each bout was separated by one week. Statistical analyses were performed by separate two‐way factorial analyses of variance (ANOVA) with repeated measures. No significant differences were observed in serum total and free T and skeletal muscle T and DHT with either protocol (p > 0.05). AR protein was significantly increased at 3 hr post‐exercise and conversely decreased at 24 hr post‐exercise for TBHV, whereas AR‐DNA binding was significantly increased at 3 and 24 hr post‐exercise (p < 0.05). Also in response to TBHV, serum Wnt4 was significantly increased at 0.5, 1, and 2 hr post‐exercise (p < 0.05) and β‐catenin was significantly increased at 3 and 24 hr post‐exercise (p < 0.05). It was concluded that neither volume of resistance exercise increased serum T, but that TBHV resistance exercise preferentially increased Wnt4/β‐catenin signaling and AR‐DNA binding that occurred independent of increases in serum and muscle androgen levels.