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Use of AMP Protein Kinase as a Potential Molecular Marker to Investigate the Development of Obesity in Non‐Model Fish Species
Author(s) -
Vides Melissa,
Evans Paige,
Kobayashi Yasuhiro
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.665.2
Subject(s) - catfish , ampk , protein kinase a , biology , messenger rna , protein subunit , gene , microbiology and biotechnology , kinase , genetics , fishery , fish <actinopterygii>
Objectives of this study were to identify the genes encoding subunits of AMP protein kinase (AMPK) in channel catfish and examine their tissue distribution. The AMPK is considered as the master regulator of tissue nutrient sensing and metabolism. Functions of AMPK have been investigated in association with obesity and diabetes development. In channel catfish, genetic selection toward growth causes development of obese‐like phenotype, suggesting channel catfish can be used as an alternative model to investigate development of obesity. However, little is known about the role of AMPK in channel catfish. In this study, genes encoding subunits of AMPK (alpha 1 and 2, beta 2, and gamma 1) were identified by comparing the channel catfish expressed sequence tag database with rainbow trout AMPK subunit mRNA. Expression of various AMPK subunit mRNA was examined in the cDNA of brain, liver, muscle, spleen, kidney and heart using RT‐PCR. Alpha 1 and 2 mRNA, as well as beta 2 mRNA, were detectable in all tissues examined. Gamma 1 mRNA expression was detectable in brain, muscle, spleen, kidney, and heart. The nucleotide sequence of amplicon corresponding to each subunit was highly similar to those of other fish (>70%). Currently, we are investigating changes in the expression of AMPK subunit mRNA in relation to changes in food intake and genetic selection toward growth in channel catfish. This study is supported by the Kansas IDeA Network of Biomedical Research Excellence.