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Analysis of PTEN and ARID1A expression in Endometriosis disease using RT‐PCR assay
Author(s) -
RosadoAlicea Jamie,
Lopez Pablo,
Flores Idhaliz,
Ortiz Carmen,
AriasDelfi Yvette,
Monteiro Janice
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.665.15
Subject(s) - pten , endometriosis , arid1a , tensin , cancer research , biology , messenger rna , endometrium , gene expression , gene , microbiology and biotechnology , medicine , endocrinology , pi3k/akt/mtor pathway , signal transduction , genetics , mutation
Endometriosis is defined as the presence of endometrial glands and stroma outside of the uterine musculature and endometrial lining. Regulation of gene expression is a flexible, yet stable, mechanism that maintains a cellular memory that could result in a disease state.The aim of this study was to analyse mRNA levels expression in endometrial tissue and endometrium from women with and without endometriosis. We will analyze mRNA transcription activity in PTEN (phosphatase and tensin homolog) , and ARID1A (AT rich interactive domain 1A) in fresh tissue from patients with endometriosis using RT‐PCR assay. In this study, we used NCBI database for PTEN and ARID1A transcript. The RNA extraction was performed using commercial kit Qiagen in endometrium and endometriosis fresh tissues. RNA integrity, quality, and purity were stringently analyzed by Experion technology. RT‐PCR analysis showed no consider PTEN and ARID1A mRNA expression in eutopic and ectopic endometrium. The expression levels of PTEN transcript (Exon5 and9) and ARID1A transcript (Exon 9 and 20) by RT‐PCR analysis were observed of mRNA expression of the PTEN and ARID1A in endometriotic tissues vs. controls. We observed that no differences statically between in eutopic and ectopic endometrium patterns of PTEN transcript P value (0.6499) and ARID1A transcript P value (0.9789) in this transcription of target genes. Our study will continue to increase more sample to improve the power analysis of the samples and provide further evidence to different patterns of PTEN and ARID1A transcription of target of these genes in endometriosis to transition to cancer.