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Strain‐dependent metabolic phenotype responses to various exercise paradigms in inbred mice
Author(s) -
Avila Joshua,
Kim Seung,
Massett Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.665.1
Subject(s) - sed , high intensity interval training , skeletal muscle , strain (injury) , medicine , endocrinology , treadmill , biology , gastrocnemius muscle , zoology
The aim of this study was to determine the interaction between genetic background and training volume (intensity x duration) on exercise performance and myogenic proteins. Male mice from four inbred strains: SJL/J, C57BL/6J, NON/ShiLtJ, and 129S1/SvlmJ completed a graded exercise test before and after four or eight weeks of treadmill training. Mice from each strain were assigned to one of three groups (n=6/group): sedentary control (Sed), continuous running (Low) (65% of max) and high‐intensity interval training (HIIT) (6 sets of 8 min at 85% of max for 8 min, followed by 2 min of low intensity at 50% of max for 2 min). Changes in exercise time were used to assess performance. Protein content of PGC‐1α, SIRT1, Glut4, Cytochrome C, Hexokinase, TFAM and NRF was examined in gastrocnemius muscle to assess the metabolic adaptation to the different intensities of exercise training. For change in time, there were significant main effects for strain, intensity and duration. There was a significant strain effect for protein content for all proteins, except PGC‐1α. Duration had a significant effect for all proteins, with protein content being greater at 8 weeks than 4 weeks. A significant intensity effect was observed for PGC‐1α and Cytochrome C with Low and HIIT being significantly greater than Sed. These data indicate that genetic background and training duration have a strong influence on adaptations to training in skeletal muscle protein. Adaptations to training in skeletal muscle proteins are not directly related to changes in exercise capacity. Supported by NIH grant HL085918 to MPM, Sydney and JL Huffines Institute for Sports Medicine and Human Performance to JJA, William Townsend Porter fellowship from the APS to JJA.