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Glia Inhibitor Blocks Ventilatory Acclimatization to Chronic Sustained Hypoxia
Author(s) -
Stokes Jennifer,
Fu Zhenxing,
Powell Frank
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.659.1
Subject(s) - hypoxia (environmental) , minocycline , microglia , saline , anesthesia , medicine , ventilation (architecture) , central nervous system , hypoxic ventilatory response , plethysmograph , pharmacology , biology , respiratory system , chemistry , inflammation , oxygen , mechanical engineering , organic chemistry , microbiology and biotechnology , engineering , antibiotics
Neuroglial communication in the central nervous system (CNS) is proving to be an important component of neuronal signaling both in normal conditions as well as stressed or injured states. We investigated the role of CNS glial cells in neural control of ventilation during chronic hypoxia. To assess this, rats received daily administration of either minocycline, a selective microglia inhibitor, (45mg/kg) or saline (i.p.) beginning one day before and during 7 days of exposure to hypoxia (PO 2 = 80 Torr). Normoxic controls, which received daily injections of either minocycline (45mg/kg) or saline, were not exposed to hypoxic conditions. Ventilation was recorded using barometric pressure plethysmography in unrestrained rats both before and after exposure to hypoxia. Daily administration of minocycline significantly (p < 0.01) decreased ventilation in normoxia and the hypoxic ventilatory response but not the hypercapnic ventilatory response, following exposure to chronic hypoxic conditions. Breathing frequency and ventilation were significantly lower in chronically hypoxic rats treated with minocycline compared to saline. Minocycline did not have an effect in normoxic conditions. Activation of microglia and astrocytes is being quantified in the NTS using immunohistochemistry. These results suggest a role for glial signaling in the physiological acclimatization during hypoxia. Supported by NIH RO1 HL‐081823