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Renal Denervation Increases Renal Blood Flow Variability in Conscious Rabbits
Author(s) -
Schiller Alicia,
Pellegrino Peter,
Zucker Irving
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.658.6
Subject(s) - denervation , renal blood flow , medicine , hemodynamics , renal circulation , beat (acoustics) , blood flow , kidney , renal artery , urology , cardiology , physics , acoustics
Activation of the renal nerves, as observed in multiple disease states, is known to have a profound impact on renal blood flow (RBF) and hemodynamics. However, little is known about their role in maintaining renal hemodynamics under normal resting conditions. We hypothesized that the renal nerves limited RBF variability. To test this, we instrumented 8 New Zealand White rabbits with radiotelemetry, and implanted a renal flow probe on the left kidney. Rabbits randomized to the denervation group underwent bilateral denervation by stripping the renal arteries of all visible fibers. In the innervated group a sham surgery was performed. Data were recorded in conscious rabbits resting quietly. Denervated rabbits had increased RBF (40.7 ± 4.2 mL/min vs. 62.0 ± 9.9 mL/min, p < 0.05) and greater beat‐to‐beat RBF variability quantified by the standard deviation of the beat‐to‐beat RBF (2.6 ± 0.2 mL/min vs 3.7 ± 0.3 mL/min p < 0.01). The increased variability persisted even after variance stabilization of the beat‐to‐beat RBF by square root transformation (0.20 ± 0.01√ (mL/min) vs 0.25 ± 0.01√(mL/min), p < 0.05). Similarly, greater dispersion of the beat‐to‐beat RBF was observed in the RBF histograms of denervated rabbits compared to innervated rabbits (p < 0.001). Frequency analysis revealed that the renal nerves decreased the proportion of high frequency variability (24.8 ± 3.8 % vs 38.5 ± 1.2 % of total power p < 0.01). Additionally, the transfer function gain between mean arterial pressure and RBF tended to be lower in innervated rabbits compared to denervated rabbits (1.00 ± 0.25 mL/min/mmHg vs. 1.89 ± 0.35 mL/min/mmHg, p = 0.08) in the high frequency range. These data indicate that the renal nerves serve to limit fluctuations in RBF. Supported by AHA PREDOC14700045.