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Effect of Adenosine on Coronary Blood Flow and Cardiac function in vivo using Adenosine Receptor Knockout Mice
Author(s) -
Teng Bunyen,
Tilley Stephen,
Ledent Catherine,
Mustafa S.
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.644.7
Subject(s) - adenosine , medicine , ventricle , cardiology , blood flow , adenosine receptor , cardiac function curve , heart rate , stroke volume , cardiac output , in vivo , anesthesia , coronary circulation , hemodynamics , receptor , blood pressure , biology , heart failure , microbiology and biotechnology , agonist
Bolus injections of adenosine (ADO) and A 2A selective adenosine (regadenoson) are used clinically as a substitute for stress test in people who can't exercise. Using different isolated tissue preparations, our lab has shown that coronary blood flow (CBF) and cardiac effects of ADO are mostly regulated by A 1 , A 2A , and A 2B ARs. In this study, we used ultrasound imaging to measure the in vivo effects of ADO on CBF (left coronary artery) and cardiac function in anesthetized WT, A 1 KO, A 2A KO, A 2B KO, A 1 and A 3 double KO (A 1 /A 3 DKO) and A 2A and A 2B double KO (A 2A /A 2B DKO) mice in real time. Echocardiographic and Doppler studies were performed using Visualsonic Vevo 2100 ultrasound system with a 30‐MHz transducer. Baseline data were obtained when animals were anesthetized by 1% isoflourane. Diameter (D) and velocity time integral (VTI) were measured on left coronary arteries of the mice (CBF=((p/4)xD 2 xVTIxHR)/1000). CBF changes were the highest within 2 min of injection (10mg/kg). Heart Rate, cardiac output, and stroke volume were measured by tracing left ventricle long axis. Data are shown below. Our data support a role for A 2 ARs in CBF and further support our conclusions of previous studies. Adenosine at this dose decreased cardiac output and stroke volume that may be A 1 , A 2B, or A 3 AR‐mediated. These data further provide a powerful translational tool in studying the cardiovascular effects of adenosine. Supported by U54GM104942; HL027339, HL094447.Figure 1Figure 2