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Coagulopathy after Polytrauma and Hemorrhage in Rats
Author(s) -
Darlington Daniel,
Wu Xiaowu,
Cap Andrew
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.641.7
Subject(s) - polytrauma , coagulopathy , medicine , intensive care medicine , anesthesia , surgery
Background Polytrauma and hemorrhage in rats leads to an elevation in Prothombin Time. This coagulopathy mimics the clinical hypocoagulation seen in both civilian and military patients after trauma. This model was used to determine if the coagulopathy is caused by a fall in pro‐coagulant or rise in fibrinolytic activity. Method: Sprague‐Dawley rats (300‐400g, n=8) were anesthetized with Isoflurane. Polytrauma was induced by damage to the small intestines, left and medial liver lobes, right leg skeletal muscle, and by fracturing the right femur. The rats were bled 40% of the blood volume. No fluid resuscitation was given. Blood samples were taken before (time 0) and 30, 60, 120 and 240min after trauma. Coagulation factors were measured in plasma by immuno or enzymatic assay. Results:Prothrombin time significantly increased over the 4hr period. Plasma thrombin activity did not change over time, however, plasmin activity and D‐Dimer concentration was significantly elevated. Tissue plasminogen activator was significantly elevated at 30mn, than fell as plasminogen activator inhibitor‐1 rose at 2‐4hrs. Anti‐Thrombin III and α‐Macroglobulin fell significantly by 2hrs. Conclusion Polytrauma and hemorrhage in rat led to a coagulopathy that was fibrinolytic as shown by the rise in plasmin activity and D‐dimers, with no change in thrombin activity. This suggests that anti‐fibrinolytic drugs like tranexamic acid may be most effective for treatment of coagulopathy after trauma. This project was funded by MRMC.