Premium
Sodium Nitrite Reduces Systolic Blood Pressure in Preeclampsia
Author(s) -
Rizzi Victor Hugo,
PossomatoVieira José Sérgio,
Mendes Gabryella,
Nascimento Regina,
Silva Katiussia,
Pupo André,
DiasJunior Carlos
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.640.4
Subject(s) - preeclampsia , blood pressure , nitric oxide , medicine , sodium nitrite , endocrinology , fetus , pregnancy , bioavailability , chemistry , pharmacology , biology , genetics , organic chemistry
Preeclampsia (PE) is a pregnancy disorder characterized by elevated blood pressure (>140/90 mmHg) and proteinuria. We developed PE in an animal model using nitric oxide synthase inhibitor L‐NAME (N ω ‐Nitro‐L‐arginine methyl ester). L‐NAME decreases the bioavailability of nitric oxide (NO) being responsible for endothelial dysfunction. Therefore reversing the reduction of NO seems to be a promising pharmacological approach. Studies showed that oral adminstration of sodium nitrite (NaNO ‐ 2 ) may increase the bioavailability of NO. Female rats (220‐250 g) (n=17) were mated with males for pregnancy. All procedures for animal experimentation were approved by the Ethics Committee, IBB/UNESP (Protocol 618/2014). From 15 th to 21 th day of pregnancy we applyed L‐NAME, (60mg/Kg, intraperitoneal ‐ i.p.) in all dams. We treated animals with NaNO ‐ 2 (n=9) (15mg/Kg, once daily, by gavage). We measured pressure by tail cuff plethysmography at gestational days 14, 16, 18 and 20. Animals were killed on pregnancy day 21 th . Systolic blood pressure was significantly reduced in animals treated with NaNO ‐ 2 (148±1.47mmHg/ 149±1 mmHg PE vs . 128±1 mmHg/ 133±1 mmHg PE+NaNO ‐ 2 , on gestational days 16 and 18, respectively). Pups body weight at birth, placentas weight and number of reabsorbed pups did not change significantly. We performed a MTT assay to verify the antioxidant conditions of plasma. MTT assay showed no difference in antioxidant status in plasma of PE and treated animals. The number of viable fetuses was significantly higher in PE+NaNO ‐ 2 (10.88±0.98 PE+NaNO ‐ 2 vs 8.25±0.64 PE). Our data showed that NaNO ‐ 2 has an antihypertensive effect and can also improve fetal viability.