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miR155 Expression is Increased by Inflammation and Modulates the Expression of CD11a in Monocytes
Author(s) -
Syed Safiya,
Amin Mohammad,
Rabquer Bradley
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.634.6
Subject(s) - microbiology and biotechnology , inflammation , monocyte , cd18 , cell adhesion molecule , cd11a , innate immune system , microrna , biology , chemistry , immunology , immune system , integrin alpha m , gene , biochemistry
Inflammation is a key process in innate immunity and helps drive the adaptive immune response. In addition, inflammatory mediators are often dysregulated in autoimmune diseases. Monocytes are key players in inflammation that reside in the blood and migrate into tissues to exert their effects. Adhesion molecules are molecules that are located on the surface of many cells and are necessary for monocyte migration. The adhesion molecule CD11a forms a complex with CD18, also known as lymphocyte function‐associated antigen‐1 (LFA‐1), and interacts with intercellular adhesion molecule‐1 (ICAM‐1) to facilitate tight binding of monocytes to the endothelium. miRNAs are known to regulate gene expression in a variety of cell types and in response to various stimuli. We hypothesized that inflammation regulates miRNA expression in monocytes and that these miRNAs play specific roles in regulating adhesion molecule expression and monocyte migration. THP‐1 monocytes were cultured, stimulated with TNF‐α, and miRNA was isolated (n=6 replicates). cDNA was prepared and qPCR was performed using primers specific for miR155, miR181a, miR181c, miR15a, miR9‐1, miR429, and miR466k. TNF‐α stimulation increased the expression of all the miRNAs, except for Mir9‐1. miRNA inhibitors were then used to assess the role of these miRNAs in regulating the expression of CD11a. After confirming inhibitor specificity and efficiency, we found that the inhibitor of miR155 reduced the expression of CD11a mRNA by 93%. In conclusion, we found that miRNAs are inducible by TNF‐α in THP‐1 monocytes and that miR155 regulates the expression of CD11a.