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A Non‐Lethal Large Animal Model of Traumatic Lung Injury (TLI)
Author(s) -
Sivieri Emidio,
Rahman Tariq,
Shaffer Thomas,
Wolfson Marla
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.632.3
Subject(s) - medicine , blunt , blunt trauma , intensive care medicine , surgery
Traumatic lung injury (TLI) affects ~25% of adult blunt trauma patients, and is a leading cause of death from blunt thoracic injury. Even those patients that avoid death after initial TLI are still at risk for multiple organ failure with survivors exhibiting acute and chronic deficits in respiratory function. Thus, it is critical to understand the underlying pathophysiological mechanisms of TLI to guide acute and chronic respiratory management. To advance the study of TLI therapeutic strategies, realistic animal models are essential to translate findings to thoracic‐injured humans. To improve standardization of high‐velocity chest trauma, this study characterizes a non‐lethal large animal (sheep) TLI model to allow the study of therapeutic intervention, acute and long‐term outcomes. The following was performed: 1) a humane bolt tunner with an integrated oscillation absorber for prevention of chest wall penetration was characterized with respect to impact dynamics (see Figure); 2) a robotic assist device was developed to off‐set gravitational and rotational forces due to the stunner weight, allowing the impact force to be precisely and reproducibly applied in all directions to the surface of the thorax; 3) the degree of closed blunt chest trauma severity was established using different charges; and 4) trauma analysis via physiologic, biochemical and histological outcomes were performed. The model achieves an advance in standardization of closed TLI and provides lung pathophysiology that parallels clinical sequelae observed in patients after high‐energy chest trauma. As such, this model closes the gap between experimental closed TLI in large animal models and clinical studies.DOD/ONR;N000141210597;N000141210810.