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Maximizing the Max Test: A Method for Determining Cardiorespiratory Fitness and Dysfunction in Mice.
Author(s) -
Petrosino Jennifer,
Mauyra Santosh,
Kalyanasundaram Anuradha,
Wilson Jacob,
Periasamy Muthu,
Ziouzenkova Ouliana
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.632.10
Subject(s) - cardiorespiratory fitness , medicine , cardiology , metabolic equivalent , physical therapy , physical activity
Early diagnostic of cardiovascular morbidity plays a critical role preventing mortality. The Bruce protocol is an exercise diagnostic test that can determine cardiorespiratory fitness. This is a standardized protocol to evaluate cardiac function in humans, yet no such standardize test has been developed for widespread use with animal models. PURPOSE The purpose of the study is to develop a similar method of testing V0 2max in mice. METHODS : Male WT mice (C57BL06, n=7) and mice with impaired cardiovascular function ( Casq2‐ null and obese WT, n=15) were used to optimize a progressive speed run test vs. our graded maximal run test and identify critical parameters indicating cardiovascular dysfunction. RESULTS Our graded test compared to the progressive lead to increased VO 2max in reduced time in obesity model. With WT and Casq2‐ null similar V0 2max was achieved in less time. Our graded test allowed for determination of a metabolic crossover parameter occurring sooner in dysfunctional animals compared to controls, as well as differential points of ventilatory threshold (VAT), time to VO 2max , and %VO 2max at VAT (P<.05). We determined that the crossover parameter is the most accurate predictor of cardiac dysfunction. CONCLUSIONS The graded exercise protocol provided valuable metabolic and cardiovascular information for fast and noninvasive evaluation of cardiovascular dysfunction in animal models.