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STUDIES ON THE SYNAPTIC FACILITATION EFFECT OF HYDROGEN PEROXIDE IN THE DEVELOPING NEUROMUSCULAR SYNAPSE
Author(s) -
Liou JauCheng,
Wu YuChu
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.621.12
Subject(s) - synapse , xenopus , neuromuscular junction , facilitation , chemistry , sodium pyruvate , hydrogen peroxide , endocrinology , medicine , biophysics , microbiology and biotechnology , neuroscience , biology , biochemistry , gene
We have previously demonstrated that muscle‐derived IGF‐1 is important in the development of neuromuscular synapse and hydrogen peroxide (H 2 O 2 ) plays an important role in the release of IGF‐1. Here we further test the role of H 2 O 2 in the development of neuromuscular synapse of Xenopus laevis by using the recording of whole‐cell patch clamp. Bath application of H 2 O 2 dose‐dependently potentiates the frequency of spontaneous ACh release in Day‐1 Xenopus neuromuscular junction. The dose‐response curve shift to left while the facilitation effect of H 2 O 2 on SSC frequency was test in Day‐3 cultures, suggesting the sensitivity of H 2 O 2 ‐induced facilitating effect reduced as synapse matured. Pretreatment of the culture with H 2 O 2 scavengers both N‐Acetylcysteine or sodium pyruvate for 1 day significantly hampered the development of synapse as the SSC frequency and amplitude both significant reduced in treated Day‐1 synapses. We next test the effect of H 2 O 2 on Day‐1 N‐Acetylcysteine/sodium pyruvate‐pretreated synapse was test after extensively washed the culture to removed N‐Acetylcysteine/sodium pyruvate. The SSC frequency facilitating effect induced by H 2 O 2 was significantly enhanced in treated synapse, which supports the notion that H 2 O 2 in involved in the development of synapse. Furthermore, co‐pretreatment the culture with insulin in N‐Acetylcysteine/sodium pyruvate group reversed N‐Acetylcysteine/sodium pyruvate treatment‐induced synapse immaturity. Overall, results from our current studies provide strong supports on the involvement of H 2 O 2 in the early development of neuromuscular synapse.