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The Intimal Alteration of Human Varicose Veins Is Associated with Oxidative Stress and Cyclooxygenase‐2 Dependent Mechanisms
Author(s) -
VelascoMartin Juan,
Aguado Andrea,
Mendez Iago,
EspañaCaparros Gabriel,
Salaices Mercedes,
Regadera Javier,
Briones Ana
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.610.1
Subject(s) - elastin , fibrosis , oxidative stress , varicose veins , intimal hyperplasia , elastic fiber , chemistry , medicine , pathology , cyclooxygenase , nadph oxidase , anatomy , surgery , biochemistry , enzyme , smooth muscle
Chronic venous insufficiency (CVI) determinates high morbidity. Main varicose histological lesions include intimal fibrosis, luminal dilation and progressive vascular wall thickening. Plasma biomarkers of oxidative stress are increased in CVI patients. We evaluated histological changes and local inflammatory profile, particularly oxidative stress and prostanoids, in varicose saphenous veins (SV). 10 distal and proximal segments of SV from patients with CVI were studied. In intimal layer, expression of smooth muscle actin (SMA), collagen I/III, and elastic fibers distribution were quantified by ImageJ sofware. NADPH oxidase subunit 4 (NOX‐4), cyclooxygenase‐2 (COX‐2), microsomal prostaglandin E synthase (mPGES‐1),MAC3, SMA, elastin, collagen I/III were studied by RT‐PCR. H 2 O 2 production and NADPH oxidase activity were evaluated by commercial kits. Histological analysis showed higher intimal expression of SMA, collagen I/III and elastic fibers in proximal segments compared to distal segments. The progressive intimal vein thickness is inversely related to the ratio of SMA, collagen I/III and elastic fibers respect to intimal area. mRNA expression of SMA, collagen I/III, elastin, NOX‐4 and COX‐2 but not of mPGES‐1 or MAC3, H 2 O 2 production and NADPH oxidase activity are upregulated at proximal varicose segments compared to distal segments. Our results suggest that oxidative stress and COX‐2 derived prostanoids might be implicated in the initial stages of the intimal fibrosis observed in varicose SV. Supported by PI13/01488 and SAF2012‐36400.