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Synergistic Effects of Phenolic Mixtures in Human Cell Models of Aging
Author(s) -
Fisher Laurel,
Ianiro Teodoro,
Lau Francis,
Wang Hong,
Daggy Bruce
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.608.36
Subject(s) - resveratrol , chemistry , polyphenol , antioxidant , biochemistry , food science , grape seed extract , oxidative stress , wine , phenols , comet assay , reactive oxygen species , dna damage , dna , medicine , alternative medicine , pathology
The health benefits associated with fruit and vegetable consumption are attributed, in the main, to fiber and micronutrients and recent research has focused on the role of phenolics (polyphenols) in mediating healthful dietary effects. While most plants contain complex mixtures of polyphenols, isolated compounds, such as resveratrol, have been widely studied and shown to influence the activity of multiple cellular metabolic and signaling pathways. Indeed, based on its actions on several cellular regulatory systems, resveratrol has been proposed to be the active molecule underlying the benefits of grape and red wine consumption. To test the hypothesis that phenolic mixtures containing resveratrol produce variable and non‐additive effects versus resveratrol alone, their antioxidant capacity (ORAC) and their effects in several in vitro models of cellular aging were compared using identical concentrations (µg phenolics/ml). Compared to resveratrol alone, the same concentration of a mixture of muscadine grape polyphenols plus resveratrol produced synergistic effects on ORAC lipophilic but not ORAC hydrophilic . Likewise, the phenolic mixture exhibited 10X greater potency versus resveratrol in inhibiting protein glycation. Non‐additive/synergistic effects (p<0.01) of the mixture versus resveratrol on mitochondrial mass, oxygen consumption and gene expression were measured in human skeletal muscle cells. Finally, synergistic effects (p<0.01) of the phenolic mixture in preventing oxidative damage to DNA (COMET assay) were observed in human pancreatic cells. These results suggest that, compared to isolated plant phenolics, the complex mixtures occurring naturally in plants have greater potency to influence various mechanisms of cellular aging.