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Sensitive Imaging Techniques Demonstrate LCPUFA‐induced Improvements of Brain Structure in Mildly Obesogenic Mice
Author(s) -
Schoemaker M.,
Arnoldussen I.,
Noordman H.,
Zerbi V.,
Wielinga P.,
Kleemann R.,
Gross G.,
Tol E.,
Kooistra T.,
Kiliaan A
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.608.10
Subject(s) - medicine , endocrinology , docosahexaenoic acid , arachidonic acid , hippocampus , context (archaeology) , polyunsaturated fatty acid , biology , fatty acid , biochemistry , paleontology , enzyme
Obesity is associated with impaired cognition. Could early nutrition with Long‐Chain Polyunsaturated Fatty Acids reduce potential detrimental effects of an obesogenic diet on brain structure in a humanized mouse model for mild obesity? Four‐week old male ApoE*3Leiden mice were fed regular chow with or without arachidonic acid (ARA) and docosahexaenoic acid (DHA). From 14 until 26 wks of age, mice received a mildly obesogenic high‐fat/high‐carbohydrate (HFHC) diet. At 13 and 26 wks of age, mice were examined in an 11.7 Tesla Bruker Biospec magnetic resonance imaging (MRI)‐scanner. GLUT‐1 fluorescent immunohistochemistry was performed. Early ARA&DHA counteracted HFHC‐induced changes in cerebral blood flow in mice at 26 wks of life, indicating improved cerebrovascular function, supported by effects on GLUT‐1 positive blood vessels. Increased mean diffusivity in the motorcortex of HFHC‐fed mice, indicative for gray matter loss, was inhibited by early ARA&DHA (P蠄0.05). Resting state functional MRI analysis showed decreased functional connectivity between ventral hippocampus, motorcortex and somasensory cortex in HFHC‐fed mice vs. control mice. Mice early fed ARA&DHA were protected against these detrimental effects. Early life ARA&DHA protects vascular function, gray matter integrity and functional connectivity between brain regions in the context of mild obesity later in life. Sensitive imaging techniques detect subtle diet effects on brain structure.