Premium
Effect of Long‐term Supplementation of Selected Medicinal Herbs on Activity of Intestinal α‐Glucosidase and Expression of SI‐complex in Rat Model
Author(s) -
Lee JungYun,
Kang YuRi,
Choi HwangYong,
Park SeungHwan,
Park Eunmi,
Kang MyungHee,
Jang HaeDong,
Kwon YoungIn
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.607.22
Subject(s) - sucrase , postprandial , carbohydrate , maltase , alpha glucosidase , acarbose , pharmacology , traditional medicine , biochemistry , biology , enzyme , endocrinology , medicine , diabetes mellitus
In this study, we investigated the effect of long‐term supplementation of selected medicinal herbs such as Cinnamomum loureirii Nees , Magnolia officinale Rehder et Wils, Brassica oleracea var. capitata L., and Ficus carica L. on activity of intestinal a‐glucosidases and expression of sucrase‐isomaltase complex (SI‐complex) in Sprague‐Dawley (SD) rat model, and also evaluated effect of herbal mixture administration on carbohydrate digestibility by postprandial blood glucose lowering test. Using 4 groups of 11 male SD rats, each group were treated with Cabagin‐S ® (positive control) and herbal mixture at low or high concentration given daily for 2 weeks. After 2 weeks administration, postprandial blood glucose lowering effect was evaluated and then small intestine biopsy was collected from each tested rat. To confirm the mechanism of action of herbal administration via regulation of carbohydrate hydrolyzing enzymes, the changes of sucrase/glucoamylase activities and expression of SI‐complex in small intestine were determined. Herbs have regulatory effects on both activity and expression of carbohydrate hydrolyzing enzymes in a similar manner with a known anti‐gastroenteric disorder drug, Cabagin‐S ® . Furthermore, our findings confirm previous in vitro observations that herbs have inhibitory effect on carbohydrate hydrolyzing enzymes, namely sucrase, maltase, and SI‐complex.