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Shikonin Prevents Adipogenic Differentiation by Inhibiting Mir‐34a
Author(s) -
Jang Young Jin,
Ahn Jiyun,
Jung Chang Hwa,
Ha Tae Youl
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.607.18
Subject(s) - adipogenesis , adipocyte , oil red o , microrna , chemistry , transfection , 3t3 l1 , mapk/erk pathway , microbiology and biotechnology , cellular differentiation , biology , biochemistry , signal transduction , in vitro , adipose tissue , gene
Shikonin is a naturally occurring naphthoquinone pigment and a major component of Lithospermum erythrorhizon. Our previous study showed that shikonin inhibited adipocyte differentiation in 3T3‐L1 cells by suppression of ERK pathway. MicroRNAs (miRNAs) are key post transcriptional regulator of adipocyte differentiation. In present study, we investigated shikonin regulates adipocyte differentiation by targeting miRNA in 3T3‐L1 cells. 3T3‐L1 cells were differentiated by 3‐isobutyl‐1‐methylzanthine, dexamethasone, and insulin (MDI) for 8 days in the presence of 0‐2 μM shikonin. We found that mir‐34a was increased during adipocyte differentiation by MDI, and was suppressed by shikonin which are measured by quantitative real‐time PCR. mRNA expression of FKBP1B, one of target of mir‐34a suggested by bioinformatics studies was decreased during adipocyte differentiation and was recovered by shikonin which are reversely correlated with mir‐34a expression. Transfection of mir‐34a inhibitor suppressed MDI‐induced adipocyte differentiation which was analyzed by Oil Red O staining, and inhibited MDI‐induced PPARγ and C/EBPα expression. Suppression of mir‐34a also recovered MDI‐induced down‐regulation of FKBP1b mRNA expression. Increase of mir‐34a by transfection of mir‐34a mimic decreased FKBP1b mRNA expression in 3T3‐L1 preadipocytes. In conclusion, these results demonstrate that shikonin prevents adipogenic differentiation by blocking mir‐34a‐FKBP1B pathway, which could be a target for obesity prevention.