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Neuroprotective effects of hexane‐ and methylene chloride‐soluble fractions of Salicornia herbacea L. in mouse hippocampal HT22 cells
Author(s) -
Kim Min Sun,
Seo Ji Yeon,
Kim Mi Hye,
Woo Ji Eun,
Kang Su Jin,
Jo Gwang Rae,
Ji HyeonKyeong,
Erdenebileg Zolzaya,
Jang Jin Ho,
Kim JaeSik,
Suh Hwa Jin,
Kim JongSang
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.607.12
Subject(s) - chemistry , hexane , methylene blue , neuroprotection , antioxidant , ethyl acetate , chloride , ethanol , fractionation , biochemistry , botany , organic chemistry , pharmacology , biology , photocatalysis , catalysis
Salicornia herbacea L. is one of the halophytic herbs that grow in salt marshes and on muddy seashores. It has been reported to exhibit their bioactivities including anti‐diabetic, antioxidant and anticancer activities. However, studies on the neuroprotective effect of this plant have not been conducted. Salicornia herbacea L. was extracted with 80 % ethanol, followed by further fractionation into five subfractions according to polarity; n ‐hexane, methylene chloride, ethyl acetate, n ‐butanol and water soluble fractions. The neuroprotective effect of plant extract and its solvent fractions was evaluated by their ability to counteract cell death and ROS generation induced by glutamate in murine hippocampal HT22 cell line. Our results indicated that n ‐hexane and methylene chloride‐soluble fractions had the highest neuroprotective activities among 5 subfractions. These fractions also induced NAD(P)H:quinone oxidoreductase, an antioxidant marker enzyme, in a concentration‐dependent manner. These results warrant further mechanistic study and identification of active compounds in n ‐hexane and methylene chloride‐soluble fractions of Salicornia herbacea L. (Supported by a grant (R0002736) from Regional Industry R&D Assistance Program (2013) of Ministry of Trade, Industry and Energy, Republic of Korea)