Premium
Evaluation of the Pathways Involved in the in vivo Biotransformation of Tyrosol into Hydroxytyrosol
Author(s) -
RodríguezMorató Jose,
Robledo Patricia,
Farré Magí,
De la Torre Rafael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.606.7
Subject(s) - tyrosol , hydroxytyrosol , wine , chemistry , in vivo , pharmacology , tyrosine , biochemistry , food science , medicine , biology , polyphenol , phenols , antioxidant , microbiology and biotechnology
Hydroxytyrosol [HT, 2‐(3,4‐dihydroxyphenyl)ethanol] is the principle phenolic compound found in olives and olive products and is also a product of dopamine metabolism. Preliminary data in humans suggest a higher recovery of HT after wine than expected (de la Torre et al. Eur J Nutr 2006;45:307). Further studies in healthy adults designed to assess the postprandial effects of moderate doses of red wine, showed that urinary recovery of HT was 4‐fold the expected based on the amount present in wine. The aims of this study were to: (1) assess the contribution of wine components to urinary HT and (2) identify the enzymes involved in this pathway. Male Wistar rats (n=96) were distributed in 14 groups (n=6‐7). The effect of tyrosine, tyrosol and tyramine on the urinary excretion (0‐4 h) of HT was assessed. We found that tyrosol was converted in vivo to HT. An additional experiment with rats (n=72) employed α‐methyl‐ p ‐tyrosine examine the involvement of tyrosine hydroxylase in this pathway. Experiments employing human liver microsomes showed that CYP450 catalyzes the conversion of tyrosol into HT. These results suggest potential mechanisms involved in the apparent excess urinary recovery of HT and contribute to the elucidation of the health beneficial effects associated with moderate red wine intake. Funded by FIS‐ISCIII‐FEDER (FIS PI14/00072), CIBEROBN (CB06/03/0028), Generalitat de Catalunya (AGAUR 2014SGR680, and Predoctoral Fellowship (FI‐DGR2012 for JRM).