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Polysorbate‐80 coated β‐carotene (encapsulated polymeric) nanoparticles accumulate in rat lungs after intravenous injection
Author(s) -
Miyazawa Taiki,
Nakagawa Kiyotaka,
Harigae Takahiro,
Onuma Ryo,
Kimura Fumiko,
Fujii Tomoyuki,
Miyazawa Teruo
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.604.8
Subject(s) - nanoparticle , chemistry , saline , microparticle , distribution (mathematics) , polymer , biodistribution , polysorbate , coating , pharmacology , biophysics , chromatography , nanotechnology , materials science , chemical engineering , biochemistry , pulmonary surfactant , medicine , organic chemistry , mathematical analysis , mathematics , engineering , in vitro , biology
Delivery of bioactive components to specific organs using nanoparticles via IV route is a recent challenge for treating and improving human health. A few studies have reported that IP administration of polysorbate‐80 (PS‐80)‐coated β‐carotene (BC) nanoparticles significantly increased BC levels in rat brain, but BC distribution into the other organs when nanoparticles are administered through IV is largely not known. To this end, we report organ distribution of BC encapsulated by biodegradable polymer (poly lactic‐co‐glycolic acid) nanoparticles after IV administration of rats. 13 weeks old Sprague Dawley rats were divided into three groups and injected with: 1) PS‐80 coated nanoparticle, 2) uncoated nanoparticle, 3) saline as control,(n=3/group). 1h after injection, PS‐80 coating was ineffective at increasing BC levels in rat brain. One theory is the nanoparticles were too large to cross blood‐brain barrier. However, PS‐80 coating caused elimination of plasma BC and high accumulation in lungs. Uncoated nanoparticle group did not show these localizations. Our results indicate that accumulation of BC in a specific organ is dependent on the nature of coating material, size of the nanoparticle, and interactions with plasma proteins. This study was supported by JSPS Postdoctoral Fellowships for Research Abroad and USDA Agreement #58‐1950‐0‐014.