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A dual functioning extensively hydrolyzed casein modulates cytokine secretion by immune cells and insulin responses from beta cells in vitro
Author(s) -
Brennan L,
Ben Larbi N,
Aarbiou J,
Gross G,
Lambers T.,
Tol E.,
Collins L,
Fischer F,
Loscher C
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.599.4
Subject(s) - immune system , cytokine , secretion , insulin , chemistry , hydrolysate , in vitro , immunology , endocrinology , biology , medicine , biochemistry , hydrolysis
Objectives Type 1 Diabetes is characterized by progressive autoimmune destruction of pancreatic β‐cells. The aim of this study was to assess whether extensively hydrolyzed casein might interfere with the release of pro‐inflammatory cytokines by immune cells and whether it might preserve β‐cell function in vitro. Methods Murine and human immune cells were pre‐incubated with specific hydrolyzed casein and fractions thereof for 1‐hour followed by activation of the cells with LPS or CD40. Cytokine secretion was measured after 24 hours. Furthermore, effects of the hydrolysate on insulin secretion by β‐cells were assessed. Results Pro‐inflammatory cytokine secretion from murine macrophages was decreased by the hydrolysate and fractions thereof. Similarly, dose‐dependent inhibition of pro‐inflammatory cytokine responses was confirmed using human primary dendritic cells and macrophages. Finally, specific hydrolysate fractions that exerted anti‐inflammatory effects also significantly promoted insulin secretion from β‐cells following exposure to IL‐1β, IFN‐γ or IL‐23. Not all hydrolysate fractions showed these activities, highlighting the potential specificity of certain hydrolysate preparations. Conclusion Extensively hydrolyzed casein and some specific fractions display dual functionality; they can exert anti‐inflammatory effects on cells of the immune system and rescue insulin secretion impaired by inflammatory activity. These mechanisms may contribute to a potential preventive effect in the development of inflammatory diseases.