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Pinolenic Acid Downregulates Anabolic Pathway of Lipid Metabolism in HepG2 Cells
Author(s) -
Lee Ah Ron,
Han Sung Nim
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.598.15
Subject(s) - lipid metabolism , palmitic acid , oleic acid , oil red o , chemistry , triglyceride , lipid droplet , anabolism , fatty acid synthesis , medicine , fatty acid metabolism , endocrinology , fatty acid , biochemistry , biology , cholesterol , gene , adipogenesis
Pine nut oil has been reported to attenuate hepatic triglyceride accumulation. However, effect of pinolenic acid (18:3, all‐cis‐Δ5,9,12), a unique component of pine nut oil, on hepatic lipid metabolism has not been studied. We investigated whether pinolenic acid affects lipid metabolism using HepG2 human liver cell line. HepG2 were incubated in serum‐free medium supplemented with 50 μM BSA (control), palmitic, oleic, γ‐linolenic, pinolenic, or α‐linolenic acids or EPA for 24h. Lipid accumulation was determined by Oil red O staining. mRNA levels of genes related to lipid synthesis (Srebf‐1c, Fasn, SCD1, ACC1), lipid oxidation (ACC2, Ppara, Cpt1), lipoprotein infusion (LDLr), and genes that may be involved in downregulation of lipogenic pathway (ACSL3, ACSL4, ACSL5) were determined by qPCR. ACSL4 protein level was measured by Western blot. mRNA levels of Srebf‐1c, Fasn, and SCD1 were significantly decreased in pinolenic acid group compared to control group (53%, 54%, and 38% less). Pinolenic acid also significantly reduced gene expression level of LDLr (43% less). ACSL3 mRNA level was significantly decreased (30% less) and ACSL4 mRNA level tended to be decreased in pinolenic acid group (20% less, P=0.082) relative to control group. In conclusion, pinolenic acid downregulated lipid anabolic pathway in hepatocyte by reducing markers related to lipid synthesis, lipoprotein infusion and regulation of lipogenic pathway. Supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (grant number NRF‐2010‐0024878).