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Rutin Reduces Body Weight with an Increase of Muscle Mitochondria Biogenesis and Activation of AMPK in Diet‐induced Obese Rats
Author(s) -
Kim Seunghae,
Seo Sangjin,
Lee MakSoon,
Jang Eugene,
Shin Yoonjin,
Oh Soojung,
Kim Yangha
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.595.4
Subject(s) - rutin , mitochondrial biogenesis , adipose tissue , endocrinology , medicine , ampk , chemistry , adipogenesis , tfam , white adipose tissue , mitochondrion , biology , biochemistry , antioxidant , protein kinase a , enzyme
Rutin is a flavonoid which exerts several beneficial properties in the health. The specific aim of this study was to investigate anti‐obesity effects of rutin in high fat diet‐induced obese rats. Male Sprague‐Dawley rats were randomly divided into 3 groups(n=8) and fed with normal diet(NOR), 45% high fat diet(HF) or 45% high fat diet containing 0.1% rutin (HF+Rutin) for 12 weeks. Compared to HF control, rutin supplementation significantly reduced body weight. Muscle mitochondrial number and size were decreased by HF, which were fully reversed by rutin. The mRNA levels related with mitochondrial biogenesis and β‐oxidation such as NRF1, Tfam, PGC‐1α, SIRT1 and CPT1 in muscle were significantly up‐regulated in HF+Rutin group. The AMPK activity was stimulated by rutin in not only muscle but also adipose tissue. In addition, rutin significantly decreased the mRNA levels of adipogenic genes such as PPAR‐γ, SREBP‐1c, and aP2 in white adipose tissue with the reduction of adipose tissue weight. Our results indicate that rutin decreased high fat diet‐induced body weight gain through PGC‐1α and AMPK activation in accompany with the decrease of adipogenesis in adipose tissue and the improvement of mitochondria function in muscle.