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Proteomic Analysis of the Low Molecular Weight Peptide Fraction in Serum of Obese Zucker Rat
Author(s) -
Hakkak Reza,
Post Steven,
Mackintosh Samuel,
West Kirk,
Tackett Alan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.595.3
Subject(s) - medicine , endocrinology , obesity , inflammation , apolipoprotein b , diabetes mellitus , fibrinogen , chemistry , tandem mass tag , cholesterol , proteomics , biochemistry , quantitative proteomics , gene
Obesity is a major risk factor for the development of cardiovascular disease, diabetes and certain types of cancer. Analysis of serum components represents a minimally invasive method for detecting biomarkers of obesity‐associated risk. Analysis of potential serum biomarkers is either focused on the levels of serum proteins or on the abundance of low molecular weight peptides that constitute the serum peptidome. In this study, we used quantitative tandem mass spectrometry to measure differences in the serum peptidome of obese Zucker rats. Lean and obese female Zucker rats (n=16) were assigned to AIN‐93‐G diet for 8 weeks, serum samples were collected and kept at ‐20 C until analysis. As expected, obese rats gained significantly (P<0.001) more weight than lean rats. Using Tandem Mass Tags (TMTs), we were able to identify and quantify a set of peptides that were elevated in the serum of obese Zucker rats. Peptides derived from apoE and the coordinately regulated apoA1, apoCIII,and apoA1 were all significantly higher in the obese rats suggesting an increase in lipoprotein metabolism. In addition, we found that fibrinogen‐derived peptides were higher in obese rats indicating enhanced inflammation. Together our results suggest that obesity induced metabolic and inflammatory changes are reflected in the serum peptidome. Supported by R01GM106024 to AJT and ABI to RH.