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Quenching of Cytotoxic Reactive Carbonyl Species by Black Rice with Giant Embryo
Author(s) -
Colzani Mara,
Lee YoonMi,
Han SangIk,
Aldini Giancarlo,
Yeum KyungJin
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.595.14
Subject(s) - chemistry , protein carbonylation , incubation , lipid peroxidation , embryo , biochemistry , in vitro , food science , enzyme , biology , microbiology and biotechnology
Reactive carbonyl species (RCS) are cytotoxic molecules originating from lipid peroxidation and sugar oxidation. A robust and efficient method based on high resolution mass spectrometry was used to test the ability of water‐soluble rice extracts to inhibit protein carbonylation induced by RCS. The model protein ubiquitin was incubated in vitro with different RCS such as 4‐hydroxy‐ trans ‐2‐nonenal (HNE) in presence of increasing concentration of various rice extracts. After 24 hours of incubation, the reaction was stopped and ubiquitin was analyzed by HRMS to determine the extent of protein modification due to the RCS, and to observe its modulation in presence of the extracts. The different rice extracts inhibited the formation of RCS‐induced protein modificationsto different extents. A black sticky rice with giant embryo (IC 50 =~29.1) strongly inhibited protein modification by the HNE than white rice (IC50=~151.0). These findings suggest that certain bioactive components in water‐soluble fractions of specific rice varieties effectivelysequester RCS, and that such rice variety can be valuable for dietary strategy for optimal health. This work was supported by Rural Development Administration (PJ010059), Republic of Korea.