Premium
Immunomodulation of innate immune cells by Sutherlandia frutescens : A transcriptomic analyses
Author(s) -
Lei W.,
Lu Y.,
Hou J.,
Li J.,
Browning J.,
Eichen P.,
Cheng J.,
Lubahn D.,
Folk W.,
Sun G.,
Fritsche K.
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.593.3
Subject(s) - stimulation , transcriptome , gene , immune system , gene expression , innate immune system , biology , downregulation and upregulation , microbiology and biotechnology , cancer research , immunology , endocrinology , genetics
Sutherlandia frutescens (SF) is a plant found in southern portions of Africa with a rich history of traditional use as a beverage to ameliorate some of the symptoms associated with cancer and HIV‐AIDS. To date no research has reported the impact of SF on global gene regulation. Therefore, we designed an experiment to evaluate the effects of SF treatment on gene expression in a murine macrophage cell line (RAW 264.7). Cells were exposed to SF extract or a vehicle control for 1 hr prior to activation with LPS and IFNγ. Gene expression at 8 & 24 hr post‐stimulation were analyzed using RNA sequencing. Immune‐stimulation caused ~2600 genes to be up‐regulated and another 2600 genes to be down‐regulated at 8 hr and at 24 hr post‐stimulation up‐ and down‐regulated genes expanded to ~4100 and >4500, respectively. SF treatment resulted in 547 differentially expressed genes (DEGs) at 8 hr post‐stimulation and for most of these genes (~64%) SF altered the direction of their expression. While the total number of genes modulated at 24 hr post‐stimulation was much greater than at 8 hr, the portion that were differentially regulated by SF treatment was lower (1369 out of 3625, ~38%). Pathway analysis identified that many of these DEGs participate in inflammatory signaling pathways, such as NF‐kB and MAPK. These findings were consistent with previously reported anti‐inflammatory activity for SF and should help us better understand the immune‐modulating properties of this botanical.