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Thermostable Alkaline Protease Production by Bacillus Aryabhattai J4
Author(s) -
Selim Samy
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.573.4
Subject(s) - protease , chemistry , hydrolysis , ultrafiltration (renal) , molecular mass , enzyme , chromatography , yield (engineering) , extracellular , biochemistry , food science , materials science , metallurgy
The current study reports the production and biochemical characterization of a thermostable alkaline protease from Bacillus aryabhattai J4, isolated from Al jouf region, KSA. The enzyme was concentrated by spinning through a centriplus, centrifugal ultrafiltration Millipore membrane with a total yield of 15%. The relative molecular mass of this protease determined by SDS‐PAGE was ranging from 40‐60 KDa. The extracellular protease of B. aryabhattai J4 was dependent on high salt concentrations for activity and stability, and it had an optimum temperature of 60 o C in the presence of 2.5 M NaCl. This enzyme was stable in a broad pH range (6‐10) with an optimum pH of 9 for azocasein hydrolysis. This extracellular protease therefore could be defined as thermostable and alkaliphilic with distinct properties that make the enzyme applicable for different industrial purposes.