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Investigating the Role of the Yeast Metacaspase, Yca1p, in the Programmed Cell Death of Aneuploid Cells of the Budding Yeast, Saccahromyces cerevisiae
Author(s) -
Rogers Stephen,
Frazier Ryan,
Pinches Seth,
Austriaco Nicanor
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.569.7
Subject(s) - aneuploidy , biology , yeast , saccharomyces cerevisiae , genome instability , programmed cell death , genetics , microbiology and biotechnology , chromosome instability , ploidy , cell cycle , mitosis , cell , apoptosis , dna damage , dna , gene , chromosome
Aneuploidy is the genetic state of a cell that has a chromosomal number that is not an exact multiple of the haploid complement. It is a leading cause of spontaneous abortions and of mental retardation in humans, and is a characteristic defect in cancer. Yeast cells that are aneuploidy manifest a diversity of phenotypes including cell cycle defects, genomic instability, protein imbalance, chaperone stress and proteotoxicity. We are investigating the links between aneuploidy on the yeast S. cerevisiae and programmed cell death. We have generated aneuploid yeast cells that either lack the yeast metacaspase, Yca1p, or contain a GFP‐tagged Yca1p, and are characterizing the behavior of these cells in response to apoptotic‐stimuli. [Our laboratory is supported by grant NIGMS R15 GM110578, awarded to N. Austriaco.]