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Bile Acids and Bile Salts of the Arabian Gulf Catfish Arius Bilineatus, Val
Author(s) -
AlHassan Jassim,
Oommen Sosamma,
Paul Bincy,
Afzal Mohammad
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.568.23
Subject(s) - glycocholic acid , cholic acid , chemistry , chenodeoxycholic acid , chromatography , lithocholic acid , bile acid , hydrolysis , deoxycholic acid , derivatization , solvent , chloroform , biochemistry , high performance liquid chromatography
Catfish gall bladders were collected from freshly caught catfish. Bile solution was collected by rupturing the gall bladder. The bile solution was freeze dried and extracted with chloroform:methanol (1:2). The solvent mixture was evaporated and the residue was extracted with methanol. The extracted material was subjected to hydrolysis to remove the conjugated parts from the bile acids and together with standards were methylated. MS analysis was performed on the methylated products. The following bile acids were obtained: Cholic acid, chenodeoxycholic acid, deoxycholic acid, and lithocholic acid. Trimethylsilyl (TMS) derivatization was done on the extracted material and analyzed by GCMS to give allocholic acid. The extracted bile salts and bile acids were also separated in LC/MS QTOF using acetonitrile: water as solvent. The following conjugated bile acids were obtained: Taurochenodeoxycholic acid, glycolithocholic acid, glycodeoxycholic acid, glycocholic acid, and cholic acid conjugated with Cysteinolic acid. (This project was funded by The Research Sector of Kuwait University Grant # SL04/09. Spectroscopic analysis was performed by Science analytical Facilities, KU).