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DHA up‐regulated expression of lipogenic and endoplasmic reticulum stress related genes in pig adipose tissues
Author(s) -
Huang ChaoWei,
Chen YuJen,
Mersmann Harry,
Ding ShihTorng
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.568.20
Subject(s) - adipose tissue , lipogenesis , endoplasmic reticulum , unfolded protein response , medicine , endocrinology , polyunsaturated fatty acid , docosahexaenoic acid , biology , chemistry , fatty acid , biochemistry
Endoplasmic reticulum (ER) is the main site for protein and lipid synthesis. In our previous study, DHA promotes triacylglycerol (TAG) accumulation in pig adipose tissues, which may correlate with ER stress. Several studies have shown the link between ER stress and lipogenesis. However, the function of n‐3 or n‐6 PUFA in regulating ER stress in adipose tissues has not been demonstrated. We determined the effect of dietary supplementation of soy bean oil (SB oil, rich in n‐6 PUFA) and docosahexaenoic acid oil (DHA oil, rich in n‐3 PUFA) on ER stress of porcine adipose tissues. 8‐week‐old weaned pigs (18.4 ± 0.3 kg) were fed with diets supplemented with 2% (as‐fed basis) of SB oil or DHA oil for 30 days with feed and water provided ad libitum . After 30 days of feeding, we measured the body compositions by EchoMRI, adipose histological section, and analyzed the expression of lipogenic and ER stress‐related genes in the adipose tissue. Body weight and body compositions were not affected by different sources of PUFA. The average size of adipocytes in DHA‐fed pigs were 1.6‐fold greater than SB‐fed pigs. Furthermore, the lipogenic related genes, CCAAT/enhancer binding protein β, hypoxia‐inducible factors and cyclooxygenase 3 were elevated by DHA treatment. Furthermore, ER‐stress related genes including activating transcription factor 6, glucose regulated protein 78 and x‐box binding protein1 are also significantly higher in DHA‐fed than SB‐fed pigs. These results indicate that DHA supplementation may increase lipogenisis and ER stress in pig adipose tissue. Whether the observed phenomena is a mechanism for animals to cope with dietary challenges awaits further investigation.