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Proteomic analysis of the interacting partners of different S6Ks isoforms.
Author(s) -
Pavan Isadora,
Freitas Lidia,
Springer Alice,
Yokoo Sami,
Carnielli Carolina,
PaesLeme Adriana,
Simabuco Fernando
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.567.6
Subject(s) - p70 s6 kinase 1 , gene isoform , biology , microbiology and biotechnology , pi3k/akt/mtor pathway , alternative splicing , rna splicing , phosphorylation , computational biology , signal transduction , gene , genetics , rna
The mTOR signaling pathway has been associated with several diseases and metabolic disorders in humans, including obesity, diabetes and several cancers. The S6Ks proteins have shown an important role in mTOR signaling, acting as effectors of this pathway. Nevertheless, the functional differences between the S6Ks isoforms is still an open question. The S6Ks family is composed of two genes, S6K1 and S6K2, encoding the isoforms p70‐S6K1, p85‐S6K1 and p54‐S6K2. The objective of this study was to investigate the interactomes related to the different S6Ks. Isoforms p70‐S6K1 and p54‐S6K2 were overexpressed in HEK293 cells, immunoprecipitated and samples submitted to mass spectrometry. The identified proteins were grouped into several classes, such as, protein synthesis, ribosomal proteins, metabolism, transcription, methylation, RNA splicing and processing, stress response, inflammation, phosphatases, cell cycle and others. Moreover, we found the known phosphorylation motif of S6Ks (RXRXXT/S) in several of these interacting partners, suggesting new possible targets of this kinase. Furthermore, the interacting partners of S6Ks were classified according to their subcellular localization. Finally, validation by western blotting was performed for some of the interacting partners. In conclusion, here we show that p70‐S6K1 and p54‐S6K2 present different interactomes, which may be related to their function in the cell.