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Urinary Candidate Biomarker Discovery in a Rat Unilateral Ureteral Obstruction Model
Author(s) -
Yuan Yuan,
Gao Youhe
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.567.2
Subject(s) - medicine , urinary system , biomarker , urine , urology , kidney disease , fibrosis , nephropathy , kidney , pathophysiology , pathology , endocrinology , biology , biochemistry , diabetes mellitus
Urine has the potential to become a better source of biomarkers. Urinary proteins are affected by many factors; therefore, differentiating between the variables associated with any particular pathophysiological condition in clinical samples is challenging. To circumvent these problems, simpler systems such as animal models should be used to establish a direct relationship between disease progression and urine changes. Obstructive nephropathy is one of the most significant causes of chronic kidney disease, and its predominant end‐stage change is irreversible interstitial fibrosis. To better detect a set of obstructive nephropathy candidate urinary biomarkers, a unilateral ureteral obstruction (UUO) model was used to observe tubular injury and the eventual development of renal fibrosis, as well as to identify differential urinary proteins in this process. In this study, urine samples were collected from the residuary ureter linked to the kidney 1 and 3 weeks after UUO to mimic urine from patients with obstructive nephropathy. Compared with the sham operation group, 7 and 19 significantly changed proteins were identified by LC‐MS/MS in the UUO 1 and 3 week groups, respectively. These proteins identified in rat models of UUO may serve as candidate biomarkers of renal tubular injury and interstitial fibrosis in obstructive nephropathy.