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Inhibition of Toxic IAPP Aggregation by Extracts of Common Fruits
Author(s) -
Moffet David,
Kao PeiYu,
Green Evangeline,
pereira Catalina,
Ekimura Shauna,
Juarez Dennis,
Whyte Travis,
Arhar Taylor,
Malaspina Bianca,
Nogaj Luiza
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.564.4
Subject(s) - amylin , amyloid (mycology) , chemistry , islet , thioflavin , in vitro , circular dichroism , protein aggregation , biochemistry , fibril , diabetes mellitus , biology , disease , alzheimer's disease , medicine , endocrinology , inorganic chemistry
Aggregation of the short polypeptide, Islet Amyloid Polypeptide (IAPP, amylin), appears to play a direct role in the death of pancreatic β‐islet cells in type 2 diabetes. Inhibiting the aggregation of IAPP has the potential to slow, if not prevent entirely, the progression of this disease. Extracts of thirteen different common fruits were analyzed for their ability to prevent the aggregation of amyloidogenic IAPP. Thioflavin T binding, immuno‐detection and circular dichroism assays were performed to test the in vitro inhibitory potential of each extract. Atomic force microscopy was used to visualize the formation of amyloid fibers with and without each fruit extract. Finally, extracts were tested for their ability to protect living mammalian cells from the toxic effects of amyloid IAPP. Several fruits were identified for having substantial ability to inhibit IAPP aggregation and protect living cells from toxic IAPP amyloid.