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Nutritional Status Modulates Box C/D snoRNP Biogenesis by Regulated Subcellular Relocalization of the R2TP Complex
Author(s) -
Houry Walid
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.562.29
Subject(s) - small nucleolar rna , biogenesis , biology , microbiology and biotechnology , nucleolus , cytoplasm , rna , genetics , long non coding rna , gene
Box C/D snoRNPs, which are typically composed of box C/D snoRNA and four core protein components (Nop1, Nop56, Nop58 and Snu13), play an essential role in the modification and processing of pre‐ribosomal RNA. The highly conserved R2TP (Rvb1‐Rvb2‐Tah1‐Pih) complex has been found to be required for box C/D snoRNP biogenesis and assembly. However, the molecular basis of R2TP chaperone‐like activity is not yet known. Here, we describe an unexpected finding in which the activity of the R2TP complex is required for Nop58 protein stability and is controlled by the dynamic subcellular redistribution of the complex in response to growth conditions and nutrient availability. In growing cells, the complex localizes to the nucleus and interacts with box C/D snoRNPs. The R2TP‐snoRNP interaction is mediated by Pih1. This interaction was found to be significantly reduced in poorly growing cells as R2TP predominantly relocalizes to the cytoplasm. Hence, the R2TP complex exerts a novel regulation on box C/D snoRNP biogenesis that affects their assembly and consequently pre‐rRNA maturation in response to different growth conditions.