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A New Look at Transcription‐Coupled DNA Repair
Author(s) -
Nudler Evgeny,
Epshtein Vitaly,
Kamarthapu Venu,
Svetlov Vladimir,
McGary Kathleen
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.490.2
Subject(s) - nucleotide excision repair , dna repair , biology , transcription (linguistics) , dna damage , dna , t cell receptor , genetics , rna polymerase , microbiology and biotechnology , gene , computational biology , rna , linguistics , philosophy , immune system , t cell
Nucleotide Excision Repair (NER) is a complex process that can detect a wide variety of DNA damage and is conserved throughout evolution. Transcription Coupled Repair (TCR) is a sub‐pathway of NER, which operates to repair the template strand of expressed genes faster than the rest of the genome. RNA Polymerase (RNAP) stalled at DNA lesions recruits NER enzymes to the damage site, suggesting an important role of RNAP in identifying DNA damage. Recently, we described a new TCR pathway, in which the NER enzyme UvrD plays the central role in assisting RNAP to initiate TCR. I will discuss the tradeoff between the new and existing TCR pathways, how and when each of them operates to repair DNA damage in E. coli, and the necessity of pervasive transcription in the maintenance of genome integrity.