Premium
The Effects of Hypogonadism and Conjugated Linoleic Acid on Regional Adipose Tissue and Fatty Acid Composition in Male Guinea Pigs
Author(s) -
Santosa Sylvia,
Yang Susan,
Weiler Hope,
DeGuire Jason,
Agellon Sherry
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.401.5
Subject(s) - adipose tissue , conjugated linoleic acid , medicine , endocrinology , lean body mass , fatty acid , testosterone (patch) , linoleic acid , composition (language) , guinea pig , chemistry , biology , body weight , biochemistry , linguistics , philosophy
Background Aging in men is associated with gains in body fat, which are due in part to the age‐related decline in testosterone. Conjugated linoleic acid (CLA) has been consistently shown to reduce body fat or fat gain in animal models through a similarly suggested mechanism as testosterone. Therefore, CLA may counteract the progressive body fat gain that occurs with testosterone decline. Objectives. To determine whether CLA modulates changes in body fat, regional fat distribution and adipose tissue fatty acid composition in hypogonadal male guinea pigs. Methods. Male, retired breeder guinea pigs (n=40, 70‐72 wk) were block randomized by weight into 4 groups: 1) SHAM/ CTRL, 2) SHAM/ CLA diet (1%), 3) Orchidectomy (ORX)/ CTRL diet, 4) ORX/ CLA diet. Dual‐energy x‐ray absorptiometry scans were performed at baseline and 16 wk to analyze body composition. Visceral and subcutaneous adipose fatty acids were extracted and methylated for gas chromatography (GC) analysis to determine fatty acid composition. Results. In ORXCTRL guinea pigs, percent body fat increased by 6.1% and percent lean mass decreased by 6.7 % over the 16 wks while no significant changes in percent body composition were observed in ORXCLA guinea pigs. Guinea pigs fed CLA diet gained less % total body fat and % upper and lower body fat than those fed CTRL regardless of surgical treatment. Regional adipose tissue of fatty acid composition showed that it was reflective of dietary fatty acids. Conclusion Hypogonadism in male guinea pigs results in increased fat mass and decreased lean mass. CLA is effective in reducing fat gain and maintaining lean mass in both hypogonadal and intact guinea pigs.