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Delivery Mode and Neonate Gut Microbiota
Author(s) -
Mueller Noel,
Pizoni Aline,
Goldani Helena,
Werlang Isabel,
Matte Ursula,
Goldani Marcelo,
Shin Hakdong,
DominguezBello M
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.385.7
Subject(s) - meconium , gut flora , bacteroides , biology , feces , physiology , phylotype , microbiome , dysbiosis , pregnancy , immunology , microbiology and biotechnology , genetics , fetus , bacteria , 16s ribosomal rna
Background C‐section (CS) babies have elevated risk of obesity, perhaps due to differences in gut microbiota during critical windows of metabolic programming. While known that the newborn gut microbiota differ by delivery mode, it is unclear when these differences emerge. Aims Here we examine how delivery mode is associated with microbiota in neonate 1 st (meconium) and 2 nd stool. Methods Our study comprises 62 neonates born by elective CS and 21 by vaginal delivery (VD) from Porto Alegre, Brazil. Bacterial V4 16SrRNA region was sequenced with the Illumina platform. Analyses were performed using QIIME. Linear discriminant analysis (LDA) effect size (LEfSe) method, which integrates statistical significance and biologic relevance, was used to compare the gut microbiota between CS and VD babies. Results In the meconium , there were no differences in microbial diversity or community structure between CS and VD babies. However, in the 2 nd stool, compared to CS babies, VD neonates had greater relative abundance of 4 phylotypes, including Bacteroides genus, and lower relative abundance of 19 phylotypes.Compared to VD babies, the 2 nd stool from CS babies also appeared to have more microbial diversity, but this difference was not statistically significant (p=0.33). Findings were similar across pregnancy weight strata. Conclusions CS babies have disrupted gut microbiota compared to VD babies, but, in our study, these differences do not emerge until the 2 nd stool. Further research is needed to elucidate what determines the microbial composition of meconium, and whether the differences in 2 nd stool microbiota by delivery mode have long‐term health consequences.