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Whole‐Wheat Supplemented Diet Changed Fecal Microbial Ecology of Obese Diabetic Mice
Author(s) -
Noratto Giuliana,
GarcíaPérez Enrique,
Ivanov Ivan,
Mills David
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.385.5
Subject(s) - firmicutes , biology , feces , bacteroidetes , gut flora , zoology , illumina dye sequencing , food science , 16s ribosomal rna , microbiology and biotechnology , genetics , bacteria , dna sequencing , immunology , gene
Diet affects bacterial diversity and abundance of the gastrointestinal microbiota. Our objective was to study the effect of whole‐wheat supplemented diet in fecal microbiota composition of obese diabetic (db/db) mice. The db/db mice were fed standard or whole‐wheat isocaloric diets for eight weeks. Fecal DNA was extracted and the V4 domain of the 16S rRNA was amplified using the universal primers F515 and R806; subsequently, amplicons were sequenced on an Illumina MiSeq instrument. Raw Illumina fastq files were demultiplexed, quality filtered, and analyzed using QIIME v1.8.0. Operational taxonomic units (OTUs) were assigned using QIIME's uclust‐based close‐reference OTU‐picking workflow, with a threshold of 97% pairwise identity against the Greengenes bacterial 16S rRNA database (13_5 release). Our results showed that the bacteroidetes/firmicutes ratio was three times higher in the whole‐wheat group compared to the db/db standard diet group. In addition, whole‐wheat diet decreased abundances of the families Enterococcaceae, Turicibacteraceae, and Ruminococcaceae. However, the alpha diversity, quantified by Shannon‐Wiener index was not significantly different between groups (p=0.0652). In conclusion, whole‐wheat supplemented diet changed the bacterial diversity and abundance of fecal microbiota of obese mice. Further studies are being conducted to analyze the relationship between microbiota changes and host metabolic responses.