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Optogenetic Manipulation of Phosphoinositides
Author(s) -
IdevallHagren Olof
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.364.1
Subject(s) - membrane , cytosol , inositol , microbiology and biotechnology , optogenetics , phosphorylation , subcellular localization , chemistry , cell , biochemistry , enzyme , biology , neuroscience , receptor , gene
Phosphoinositides, the phosphorylated products of inositol phospholipids, play critical regulatory roles in cell physiology. Through interactions mediated by their cytosolically exposed and differentially phosphorylated head groups they help control membrane‐cytosol interactions. As each of the 7 phosphoinositide species has a unique subcellular localization, they also help define specific interaction of different membranes. The elucidation of phosphoinositide functions greatly benefit from methodologies to manipulate their concentrations within specific membranes with high spatial and temporal precision. The talk will discuss the use of the blue light‐dependent CRY2‐CIB1 dimerization modules (1,2) to rapidly and reversibly relocate phosphoinositide‐metabolizing enzymes to control specific phosphoinositide dependent reactions within cellular membranes.