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Signals that Distinguish Noncoding RNA Scaffolds from mRNAs
Author(s) -
Moazed Danesh,
Iglesias Nahid,
Sevilimedu Aarti,
Yu Ruby,
Jih Gloria,
Kruger Ryan,
Gygi Steven
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.361.2
Subject(s) - heterochromatin protein 1 , heterochromatin , euchromatin , polyadenylation , microbiology and biotechnology , biology , rna interference , histone , chromatin , constitutive heterochromatin , rna , genetics , dna , gene
At the fission yeast pericentromeric DNA repeats, nascent noncoding transcripts act as scaffolds for the siRNA‐programmed RITS complex, which then recruits histone H3 lysine 9 (H3K9) methylation and promotes heterochromatin formation. However, siRNAs cannot promote heterochromatin formation at most complementary euchromatic regions, suggesting that mRNAs are poor scaffold siRNA‐mediated H3K9 methylation. We recently showed that cleavage and polyadenylation signals within the 3′ untranslated region of an mRNA antagonize siRNA‐mediated heterochromatin formation. Consistent with these results, we find that the recruitment of a conserved nuclear deadenylation complex to target RNAs is critical for RNAi‐mediated heterochromatin establishment. These results suggest that transcription termination signals and alternative polyadenylation pathways determine whether a nascent transcript can act as a scaffold for the recruitment of chromatin‐modifying complexes.