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Biogenesis and Mechanism of Action of Enhancer RNAs
Author(s) -
Shiekhattar Ramin
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.361.1
Subject(s) - enhancer , enhancer rnas , biogenesis , microbiology and biotechnology , biology , integrator , transcription (linguistics) , regulation of gene expression , gene expression , gene , genetics , physics , linguistics , philosophy , quantum mechanics , voltage
Enhancers are distal regulatory elements that mediate tissue‐ and temporal‐specific gene expression during development. Recent evidence has revealed that active enhancers are transcribed and that such enhancer RNAs (eRNAs) play an important role in transcriptional activation. We show that the metazoan‐specific RNA polymerase II (RNAPII)‐associated complex, Integrator, is recruited to enhancers and super‐enhancers in a stimulus‐dependent manner. Functional depletion of Integrator subunits diminished the signal‐dependent induction of eRNAs at enhancers and super‐enhancers. Global nuclear run‐on and RNAPII profiling demonstrated that Integrator regulates the maturation (3′‐end processing) of eRNAs. We show that the production of mature eRNAs require the catalytic activity of Integrator complex. We propose a role for Integrator in biogenesis of eRNAs in metazoans.