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A Cholesterol Story Never Told before
Author(s) -
Cho Wonhwa,
Sheng Ren,
Liu ShuLin
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.357.3
Subject(s) - cholesterol , cell , microbiology and biotechnology , function (biology) , cell signaling , biology , computational biology , signal transduction , cell function , chemistry , biochemistry
Cholesterol is known to interact with various cellular proteins but its direct involvement in cell regulation has not been fully demonstrated. To systematically investigate the regulatory function of cholesterol, we have taken a two‐pronged approach of identifying cellular cholesterol binding proteins and developing a quantitative in situ imaging analysis of cholesterol. Through genome‐wide screening of domains and proteins we have identified many cholesterol‐binding cellular proteins and elucidated how cholesterol specifically regulates key cell signaling pathways via these proteins. Our new quantitative imaging analysis has also revealed strikingly unique spatiotemporal distribution and dynamics of cholesterol in mammalian cells, which is crucial for its specific regulation of cell signaling pathways.