Premium
Integrated Transcriptomic, Proteomic, and MicroRNA Data Identify Key Regulators of Developmental and Repair Processes During Tail Regeneration in the Green Anole Lizard
Author(s) -
Hutchins Elizabeth,
Eckalbar Walter,
Pendarvis Ken,
McCarthy Fiona,
Lake Douglas,
Kusumi Kenro
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.346.3
Subject(s) - biology , transcriptome , microrna , regeneration (biology) , wnt signaling pathway , microbiology and biotechnology , proteome , gene expression , gene , computational biology , genetics , signal transduction
Among amniotes, mammals and birds display only limited ability for regeneration in the adult. However, lizards retain the ability to lose their tails and regrow a functional replacement that includes newly formed hyaline cartilage, spinal cord, muscle, vasculature, and skin. These newly regenerated tails, which have an organization distinct from the original tail, are formed through a complex process that involves differential expression of hundreds of genes, including those related to wound healing, musculoskeletal development, hormonal response, embryonic morphogenesis, and Wnt pathway activation. We performed whole transcriptome, microRNA transcriptome, and proteome analysis of regenerating tail tip and base and associated tissues in order to investigate gene expression in regenerating tissue. We identified 350 novel and 196 known microRNA precursor families and over 2,000 proteins. Differentially expressed microRNAs in the regenerating tail include miR‐133a, miR‐133b, and miR‐206, which have been reported to regulate regeneration and stem cell proliferation in model systems, as well as three novel microRNAs. Additionally, we integrated microRNA and mRNA expression patterns with proteomic analysis in order to confirm the presence of predicted targets of down‐regulated microRNAs. Using a combination of whole and microRNA transcriptome with proteome data, we have identified key microRNA regulators and their targets that control the regenerative process in the green anole lizard, which has value for translation to future regenerative therapies.