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DHA‐enriched high oleic canola oil diet may accelerate the cholesterol synthesis in humans.
Author(s) -
Jones Peter,
Pu Shuaihua,
RodríguezPérez Celia,
Ramprasath Vanu,
Khaloufi Khatima,
Sabra Ali,
SeguraCarretero Antonio
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.248.4
Subject(s) - lathosterol , canola , desmosterol , campesterol , cholesterol , oleic acid , chemistry , food science , sterol , crossover study , soybean oil , zoology , biochemistry , biology , medicine , placebo , alternative medicine , pathology
Circulating plant sterols are commonly used as surrogate biomarkers of cholesterol absorption, while desmosterol and lathosterol levels indicate cholesterol synthesis. To investigate the health benefits of various dietary oils varying n‐9/n‐6 fatty acid profiles on cholesterol trafficking using markers of cholesterol absorption and synthesis, a double‐blinded randomized crossover clinical trial consisting of five 30‐d periods was conducted. Diets included 60 g/d of canola oil (Canola, 59% OA, 20% LA, 10% ALA), high oleic canola oil (CanoalOleic, 72% OA, 15% LA), high oleic canola/DHA oil (CanolaDHA, 63% OA, 13% LA, 6% DHA), corn/safflower oil (CornSaff, 18% OA, 69% LA) and flax/safflower oil (FlaxSaff, 18% OA, 38% LA, 32% ALA). Total cholesterol and other sterol measures were determined using GC‐FID at endpoints. Results (n=54) showed that CanolaDHA feeding produced the highest serum total cholesterol levels (p<0.05) compared to other four treatments. No differences were observed in desmosterol levels across all diets. CanolaDHA also produced the highest lathosterol (p<0.0001) among all treatments with 27% more than the n‐6 control CornSaff. The sum of sitosterol and campesterol levels within three n‐9 rich diets, Canola, CanolaDHA, and CanolaOleic were higher (p<0.001) than two n‐6 rich diets by 27%, 34% and 20%, respectively. The data suggest that dietary DHA oil may accelerate cholesterol synthesis, resulting in high circulating cholesterol levels while n‐9 rich diets can elevate cholesterol absorption compared to n‐6 rich diets.